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Fluorescence microscopy
———————–
*C. albicans* was grown in a single day at 37°C in 5 ml of YPD medium. Cells had been transferred to a brand new 10 ml YPD tradition tube after which incubated for six h at 37°C. Samples had been then transferred to Nunc Lab-Tek 2 chamber slide and smeared on glass utilizing a small scalpel. The slide was dried, mounted by immersion in methanol at -20°C for 1 min after which air-dried. Fungal cells had been stained by incubation with DAPI (1 μg/ml) in PBS for 30 min after which rinsed with PBS. The slide was air-dried and saved at 4°C. To visualise cells, the stained slide was positioned at midnight on a glass slide and examined by fluorescence microscopy on Zeiss (543 nm for excitation and 580 nm for emission).
**Calcofluor-white staining**
—————————–
An aliquot of 100 μL of *C. albicans* in 30% glycerol was combined with calcofluor (Sigma, USA), 5 μL of which was then added to the wells of a 24-well plate containing 300 μL of molten YPD agar at 37°C. The plate was incubated at 37°C, after which the outcomes had been visualized utilizing a fluorescence microscope.
**
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